Prevalence of Bacterial Leaf Spot of Bottle Gourd and Pumpkin in Subtropical Zone of Himachal Pradesh

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Kumud Jarial
Sujata Kumari
R. S. Jarial
Savita Jandaik
Deepa Sharma


To assess the prevalence and severity of bacterial leaf spot on bottle gourd and pumpkin, a survey was conducted in Hamirpur, Una and Bilaspur districts of sub tropical zone of Himachal Pradesh, India during the years 2018 and 2019. Data were recorded in terms of disease severity and fruit rot incidence. The associated pathogen from bottle gourd and pumpkin was isolated on nutrient sodium chloride agar medium and identified on the basis of morphological, biochemical and pathogenicity tests on bottle gourd and pumpkin seedlings. Disease was found to be prevalent at all the locations surveyed exhibiting a mean disease severity from 24.70 to 87.55 and 5.30 to 52.92 per cent in bottle gourd and pumpkin, respectively. Fruits of bottle gourd were recorded to be affected badly exhibiting a mean fruit rot incidence of 10.23 to 95.32 to per cent, while, no fruit rot incidence was recorded in pumpkin fruits. The colonies of the isolated bacterium were mucoid, circular, smooth textured and yellow in colour having a diameter of 2-4 mm. The pathogen was found to be Gram–ve and tested positive for esculin hydrolysis as well as protein digestion test. During pathogenicity tests, incubation period of 2 and 4 days was recorded on bottle gourd and pumpkin, respectively. Based on these studies, the identity of the pathogen was confirmed to be Xanthomonas cucurbitae(ex Bryan) Vauterin et al.

Xanthomonas cucurbitae, occurrence, pathogenicity, bacterial leaf spot, bottle gourd, pumpkin.

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How to Cite
Jarial, K., Kumari, S., Jarial, R. S., Jandaik, S., & Sharma, D. (2020). Prevalence of Bacterial Leaf Spot of Bottle Gourd and Pumpkin in Subtropical Zone of Himachal Pradesh. Journal of Scientific Research and Reports, 26(9), 10-19.
Original Research Article


Ajuru M, Nmom F. A review on the economic uses of species of cucurbitaceae and their sustainability in Nigeria. Am J Plant Biol. 2017;2(1):17-24.

Jarial K, Dogra BS, Mandradia RK, Kumar S, Sharma D, Gupta AK. Investigations on a new bacterial disease of bottle gourd in sub-tropical zone of Himachal Pradesh. Plant Dis Res. 2011;26(1):68-75.

Babadoost M, Ravanlou A. Outbreak of Bacterial Spot (Xanthomonas cucurbitae) in Pumpkin Fields in Illinois. Plant Dis. 2012;96:1222.

Bryan MK. Bacterial leaf spot of squash. J. Agric. Res. 1930; 40(4):385-391.

Ryan RP, Vorhalter FJ, Potnis N, Jones JB, Van Sluys M, Bogdanove AJ, Maxwell J. Pathogenomic of Xanthomonas: understanding bacterium-plant interaction. Annu. Rev. Phytopathol. 2011;9:344-436.

Doidge EM. A tomato canker. Ann App Biol. 1921;7(4):407–30.

Dowson WJ. On the systematic position and generic names of the Gram-negative bacterial plant pathogens. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1939; 100:177–193.

Kado CI, St Paul MN. Plant Bacteriology. The American Phytopathological Society. 2010;336.

Vauterin L, Hoste B, Kersters K, Swings J. Reclassification of Xanthomonas. Int J Syst Bacteriol. 1995;45:472-489.

Larazev AM. Diseases: Xanthomonas campestris pv.cucurbitae (Bryan) dye-bacterial leaf spot of cucurbits in: 2003-2009 Project “Interactive agricultural ecological atlas of Russia and neighbouring countries: Economic plants and their diseases, pests and weeds”. 2009; Accessed 17th October, 2020. Available:

Salamanca LR. Bacterial diseases of pumpkins: An old enemy and an emerging bacterial disease. Michigan State University Extension Bulletin; 2014. Accessed 15th July, 2019. Available:

McKinney HH. Influence of soil temperature and moisture on infection of wheat seedlings by Helminthosporium sativum. J Agric Res. 1923;26:195-217.

Society of American Bacteriologists. Mannual of microbiological methods. New York, McGraw Hill Book Company. 1957;315.

Schhad NW, Stall RE. Xanthomonas in: Laboratory guide for identification of plant pathogenic bacteria by N.W. Schaad (ed.). American Pythopathogical Society, St. Paul, Minnesota. 1988;81- 94.

Aneja KR. Experiments in microbiology, Plant pathology and biotechnology. 4th revised edition. New Age International (P) Limited, Publishers. 2015;607.

Sharma RR. Integrated management of bacterial spot of bottle gourd caused By Xanthomonas cucurbitae. M.Sc. Thesis. Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu main campus, Chatha, Jammu-180009 INDIA. 2016;81.

Altin I, Casoli L, Stefani E. First report of bacterial spot caused by Xanthomonas cucurbitae on pumpkin in Italy. New Dis Rep. 2020;41:21. Available:

Lamichhane JR, Varvaro L, Balestra GM. Bacterial leaf spot caused by Xanthomonas cucurbitae reported on pumpkin in Nepal. New Dis. Rep. 2010; 22:20.

Dutta B, Gitaitis RD, Lewis, KJ, Langston DB. A new report of Xanthomonas cucurbitae causing bacterial leaf spot of watermelon in Georgia, USA. Plant Dis. 2013;97:556.

Ravanlou A, Babadoost M. Development of bacterial spot, Incited by Xanthomonas cucurbitae, in Pumpkin Fields. Hort. Sci. 2015;50(5):714-720.

Kumari S, Jarial K, Jarial RS, Jandaik S. Cultural parameters of Xanthomonas cucurbitae causing bacterial leaf spot of bottle gourd and pumpkin. Int J Biores Str Manage. 2020;11(3):214-218.

Zhang X, Babadoost M. Characteristics of Xanthomonas cucurbitae isolates from pumpkins and survival of the bacterium in pumpkin seeds. Plant Dis. 2018;102(9): 1779-1784.